MF contributed to the analysis of the microsatellite data. Scoring microsatellite loci. 2015;282:20141850. R Core Team. In these systems separation has already proceeded which prevents the identification of the earliest barrier. We tested the reaction of about 20 female wasps for each strain. In a four-chamber olfactometer we tested for the presence of a positive reaction to the host habitat odour of bird nests in nine nest and 10 carrion strains, and for the presence of a positive reaction to carrion odour in 11 nest strains and 10 carrion strains (Additional file 1: Table S1). Gordh G, DeBach P. Courtship behavior in the Aphytis lingnanensis group, its potential usefulness in taxonomy, and a review of sexual behavior in the parasitic Hymenoptera (Chalcidoidea: Aphelinidae). Mitchell, T. B. 2002;17:5207. Correspondence to Hybrid breakdown between two haplodiploid species: the role of nuclear and cytoplasmic genes. Contrasting signatures of genomic divergence during sympatric speciation. 2017;90 Supplement C:2333. We studied ecological barriers, sexual barriers, and the reduction in F1-female offspring as a postmating barrier, as well as the population structure using microsatellites. For each barrier, we used the absolute contribution (AC). Because we observed no differences in sex ratio in all experiments on postmating isolation, DM incompatibilities and CI of any type is unlikely as explanation. wing morphology [67], circadian rhythm and diapause [68, 69], chemical communication [64, 70], sex determination [71], evolution of venom [72], and learning [73, 74]. Hymenoptera display an array of interesting behavioral characteristics, particularly in social species. 2020;287:20201107. a science and as a profession, encourages research relative to insects and related Abraham R, Peters RS. Schurmann D, Sommer C, Schinko AP, Greschista M, Smid H, Steidle JL. Ancestral state reconstruction analysis of hymenopteran sex determination mechanisms. Bait bags were made of aluminium gauze (4.5cm1cm, 1.5mm mesh width) and contained five fly pupae of the green blowfly Lucilia sericata (Meigen 1826) (Diptera: Calliphoridae). In: Speciation: natural processes, genetics and biodiversity. moths and butterflies. One bait bag was also placed next to each of 15 steel grid cages (21511555mm) containing fresh carrion of small rodents (Mus musculus L., Microtus arvalis (Pallas 1778), Meriones unguiculatus (Milne-Edwards 1867)). Our study confirms the existence of two different ecotypes in N. vitripennis, one occurring in bird nests, and the other in carrion. The cuckoo bee (Anthophorinae), a close relative of the bumblebee, lays its eggs in bumblebee nests, where the larvae are cared for and nourished by bumblebee workers. Because this revealed no difference between strains within each group (nest or carrion), data between nest and carrion strains were compared using non-parametric MannWhitney U-test. For each strain, about 20 females were tested for their response to nest and carrion odour. Collectively, the Hymenoptera are most important to humans as pollinators of wild and cultivated flowering plants, as parasites of destructive insects, and as makers of honey. Every chamber contains a glass dish ( 40mm, height 7mm). Sympatric speciation in phytophagous insects: moving beyond controversy? Now, the debate has shifted to the question, how frequently sympatric speciation occurs [7]. The fire ant (Solenopsis saevissima), accidentally introduced into the United States from South America, feeds on young plants and seeds and is known to attack young mammals. The main habitats are bird nests for subpopulation 1 and carrion for subpopulations 25. Loci on all five chromosomes of N. vitripennis were chosen to achieve a better coverage of the genome and to account for different selection strengths between chromosomes (Table 7). For test statistics see Tables 3 and 4. However, its larger host range comprising pupae of blowflies, fleshflies, houseflies, and others [126] suggests that it is a generalist. 1990. 1989;2:26583. Host pupae had been killed by freezing at 20C and subsequently thawed at room temperature (see above) to rule out potential differences between the strains based on any ability to suppress the host immune defence. First of all, we will look for a few extra hints for this entry: Wasp-like, hymenopterous fly whose larvae feed on other live insect larvae. Social species may live in colonies of as many as 1,000,000 individuals. The fact that all other species of the genus Nasonia occur in bird nests [126] could indicate that N. vitripennis has originated from a bird nest specialist. Interestingly, this hypothesis is analogous to the idea that the high diversity in the ambrosia beetle tribe Xyleborini results from haplodiploidy and reduced gene flow between lineages due to close inbreeding [42]. 2008;180:1593608. Science. Behavioural and genetic analyses of Nasonia shed light on the evolution of sex pheromones. The parasitoid wasp Nasonia: an emerging model system with haploid male genetics. Total isolation (T) was calculated using the following formula: A population genetic analysis was performed with 103 females to study the population substructure. A 24-h survey in 2013 (Geo-Tag der Artenvielfalt) revealed the presence of at least 365 plant species and 846 animal species in the area. Most species, however, are solitary in habit. Multiple comparisons were performed with post-hoc Tukeys test [158]. Conner JK, Hartl DL. Entomol Exp Appl. Kautt AF, Kratochwil CF, Nater A, Machado-Schiaffino G, Olave M, Henning F, et al. Different lower case letters indicate significant differences (p<0.05, generalized linear models with family quasipoisson followed by ANOVA and Tukey-test. These movements were carefully studied and described by the German entomologist Karl von Frisch. In bird nests, wasps were present from May until end of July after nestlings had left, while they occurred on carrion from May until September. 2005;8:33652. We acknowledge Elders past, presentandemerging. Peer K, Taborsky M. Outbreeding depression, but no inbreeding depression in haplodiploid ambrosia beetles with regular sibling mating. Endosymbiotic Rickettsiella causes cytoplasmic incompatibility in a spider host. PubMed Central 2005;102(suppl 1):655865. Google Scholar. frequency of mating or the number of offspring after mating), while C refers to events, which enable gene flow between different inbreeding lines, microsatellite clusters or ecotypes. https://doi.org/10.1186/s12862-021-01938-y, DOI: https://doi.org/10.1186/s12862-021-01938-y. 2001;409:70710. Armies of as many as 1,500,000 such insects destroy almost all animal life they encounter. Nearly all commonly encountered Hymenoptera can be recognized by a narrow "waist." Principles of population genetics. To calculate RI for sexual isolation, we used the data of the mating experiments presented here. J Evol Biol. https://doi.org/10.1126/science.1178028. You have reached the end of the page. CAS The mouthparts may be either of the biting type or of the biting-sucking type. The most colorful of bees are a group of Tropical American insects called Orchid Bees, which have brilliant iridescent colors of green, blue and red. Inbreeding shapes the evolution of marine invertebrates. 2019. http://www.nhm.ac.uk/chalcidoids. The Rate of evolution of postmating-prezygotic reproductive isolation in Drosophila. Clark ME, OHara FP, Chawla A, Werren JH. There was no difference between couples from the same strains and couples from different strains but from the same microsatellite cluster with k=3 subpopulations. Higashi M, Takimoto G, Yamamura N. Sympatric speciation by sexual selection. The Belknap Press of Harvard University Press, Cambridge. Sequential sympatric speciation across trophic levels. J Zool Syst Evol Res. The honeybee has been valued since pre-Christian times for its honey and beeswax. Then, single females were transferred into Petri dishes (6015mm) with 10 pupae of L. sericata for parasitization and kept there until their death. Google Scholar. The Hymenoptera (bees, wasps and ants) occur in almost all terrestrial ecosystems in large species numbers where they often play a fundamental ecological role as herbivores, pollinators, or natural enemies [23]. Host race formation and sympatric speciation in Rhagoletis fruit flies (Diptera: Tephritidae). 2018;72:25715. Sympatric speciation in the post Modern Synthesis era of evolutionary biology. The habitat of origin had a strong effect on the reaction of the wasps. Based on these and other traits, the ecotypes meet many criteria for host races of plant feeding insects [14, 112, 113]: They are associated with different habitats and hosts which is demonstrated by their specific reaction to habitat odours, their host acceptance and possibly their phenology. 2007;99:27887. Nature. A new parasitoid wasp, Aphaereta vondelparkensis sp. Taken together, the data demonstrate that our local population of N. vitripennis is structured on three levels: Between inbreeding strains, between genetic clusters based on microsatellite analysis, and between ecotypes. To identify the most likely of these hypothetical scenarios, an analysis of the ecology and the population genetics of N. vitripennis populations from a larger geographic area, e.g. 2010;35:6776. Genetic structure of natural Nasonia vitripennis populations: validating assumptions of sex-ratio theory. Therefore, the actual number is probably much higher. Nature. Single comparisons were made using Tukey-test based on linear mixed models or generalized mixed models with field as factor and observation as random factor (Table S2, separate file). Principal differences in structure within the order include the presence or absence of wings that, when present, consist of two pairs; the presence or absence of a waist; modifications of the ovipositor; and adaptations of mouthparts for specific eating habits. The honey ant (Myrmecocystus in the United States, Plagiolepis in Africa) has in the nest a division of worker ants known as repletes, which are fed sugary secretions. Putative population structure of the parasitoid N. vitripennis in the Hohenheim Park (Germany) based on Delta K. Results from three microsatellite analyses (Run 1-Run 3) using STRUCTURE v2.3.4 [159], and the online tool CLUMPAK. Proc Natl Acad Sci. 2). Frankham R, Briscoe DA, Ballou JD. Postzygotic isolation evolves before prezygotic isolation between fresh and saltwater populations of the Rainwater Killifish, Lucania parva. J Stat Softw. These conditions were used for all cultures. Geuverink E, Rensink AH, Rondeel I, Beukeboom LW, van de Zande L, Verhulst EC. Smithsonian Institution Press, Washington and London. Please refer to the appropriate style manual or other sources if you have any questions. Bouek Z, Rasplus JY. F1-female offspring numbers from experiments on postmating barriers were compared by using generalized linear models, quasipoisson and negative binomial, followed by ANOVA and post-hoc Tukeys tests [158]. Inbreeding and outbreeding. Many species of Hymenoptera are extremely small and are thus difficult to identify even to family. 1999;41:2937. 2010;104:289301. While CI in Nasonia is caused by different strains of Wolbachia, which is the best studied reproductive manipulating endosymbiont [81], there are at least three other endosymbionts which are able to cause the same effect [82,83,84,85]. 1993;142:73754. 1993;135:56574. Aguiar AP, Deans AR, Engel MS, Forshage M, Huber JT, Jennings JT, et al. While a high level of within patch inbreeding was also found in a Swedish population of N. vitripennis [125], the existence of subpopulation clusters within a local population has not been reported before for N. vitripennis. Sexual isolation was studied with wasps from strains originating from bird nests and carrion (strains from bird nests: N2, N3, N9; strains from carrion: A1, A7, A19). Knig K, Zundel P, Krimmer E, Knig C, Pollmann M, Gottlieb Y, et al. However, the observed pattern could also be the result of immigration of ecotypes that evolved their adaptations in allopatry (Fig. All authors read and approved the final manuscript. The Wasps. Noyes JS. Demonstration of long-term memory in the parasitic wasp Nasonia vitripennis. CAS Generalized linear models (family poisson) followed by ANOVA revealed no difference between strains within each group (nest or carrion). Ovipositors of Hymenopteta are usually well developed and modified into a stinger in the higher forms of the order. After incubation at 56C, the solution was washed in repeated steps with buffer solutions and subsequent centrifuging. intermediate stages between polymorphic populations and full species [14], are taken as evidence that genetically differentiated populations can exist despite gene flow which indicates that sympatric speciation is common [12, 14].

Order Hymenoptera. Experimental wasps were dissected from the host to exclude experience as described above and kept in groups of 1020 individuals in perforated Eppendorf tubes prior to the experiments. Russ J Genet. 8, scenario C). van Achterberg K, Schilthuizen M, van der Meer M, Delval R, Dias C, Hoynck M, et al. Benefits to mankind. Catalog of Hymenoptera in America North of Mexico. on the level of ecotypes. Strain abbreviations with N refer to strains collected in bird nests, strains with A were collected next to carrions. PubMed Sex ratio evolution in a variable environment. CAS 1). We have 1 possible solution for this clue in our database. 2017;82:126. Male development CI would result in an increased number of male offspring and no change in the total number of F1-offspring. Wiegmann BM, Mitter C, Farrell B. 2009;2009:pdb.emo134. To study phenology and to establish laboratory cultures of N. vitripennis, wasps were collected using bait bags with fly pupae in the park of the University of Hohenheim (Stuttgart, Germany). Reproductive isolation among allopatric Drosophila montana populations. Thereby the adaptation to the microhabitats could be based on new mutations, or on ancestral alleles present in low frequencies in the original population [127]. They are based mainly on genetic incompatibilities in nuclear-nuclear or nuclear-mitochondrial interactions according to the DobzhanskyMuller model [59, 62, 79], and on endosymbiont induced cytoplasmic incompatibility (CI). Hybrid speciation. Zootaxa. the reaction of wasps from strains A1, A7, and A19 to odours from carrion, and their higher acceptance of carrion fly pupae would have evolved independently. Privacy Butcher BA, Smith MA, Sharkey MJ, Quicke DL. Peters RS, Krogmann L, Mayer C, Donath A, Gunkel S, Meusemann K, et al. Steidle JL, van Loon JJ. BMC Genomics. Schurmann D, Kugel D, Steidle JL. 2011;11:518. volume21, Articlenumber:204 (2021) of F1-female offspring from couples consisting of females and males from different strains. Remarkably, these ecotypes consist of different microsatellite subpopulations. 1995;49:70517. However, it has never been explicitly studied. 1969;62:3058. Van Oosterhout C, Hutchinson WF, Wills DP, Shipley P. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Oxford: Oxford University Press; 2012. 2002;17:23041. Generally, the reduction in F1-female offspring in couples with partners from different female strains and microsatellite clusters can have different reasons: (1) Genetic incompatibilities according to the DobzhanskyMuller (DM) model due to nuclearnuclear or nuclearmitochondrial interactions, which are both described for Nasonia [59, 62, 79]. 2009;96:38391. 1978;46:3775. Maan ME, Seehausen O. Ecology, sexual selection and speciation. It was calculated as follows: AC for the second barrier: AC2=RI2*(1AC1). The most detailed studies on the evolution of reproductive barriers exist for the genera Lariophagus (Chalcidoidea: Pteromalidae), parasitoids of beetle larvae [52, 57, 58], and Nasonia (Chalcidoidea: Pteromalidae), parasitoids of fly pupae in bird nests or on carrion [59,60,61,62,63,64,65]. He suggested that it could increase reproductive isolation of lineages by genetic drift [40, 41], and promote speciation similar to geographic barriers in allopatric populations. The nest ecotype is mainly formed from one of the microsatellite clusters, the two or four remaining microsatellite clusters form the carrion ecotype. The number of F1-male offspring in the treatments with partners from different microsatellite clusters or different microhabitats were always slightly lower (Fig. 2010;10:109.

numbers of parasitized pupae and offspring did not have a normal distribution, the differences between strains were analysed using generalized linear models (family poisson) [157]. 2009;9:132232. (3) A lower reproductive investment by females after matings with males from a different strain. The other ecotype attacks only carrion flies (genera Calliphora, Lucilia etc.) Forbes AA, Bagley RK, Beer MA, Hippee AC, Widmayer HA. 1996;263:17039. Ecological isolation as a premating barrier was studied with respect to phenology, differences in the reaction to olfactory host habitat cues, and host acceptance. Host acceptance was tested with pupae of L. sericata carrion flies (Fig. Likewise, females produced more F1-female offspring after copulations with males from the same microsatellite cluster as compared to copulations with males from a foreign cluster. Ecotypes are separated only by ecological barriers. Chemoecology of parasitoid and predator oviposition behaviour. The number of F1-female offspring was significantly influenced by the origin of mating partners from the same strain, from different strains but the same microsatellite cluster with k=3 or k=5 subpopulations, or the same microhabitat (Fig. A maximum of five females from each strain were tested per day. Subpopulation 2 was excluded from the analysis with k=5 due to the small number of individuals. Rosenwald LC, Sitvarin MI, White JA. The evolution of reproductive isolation in Daphnia. 2010;79:77784. A Females and males originate from the same strain (green), or from different strains but the same microsatellite cluster with k=3 (yellow) or k=5 (orange) subpopulations, or the same microhabitat (blue). MP and VP performed the olfactometer experiments. As mentioned above, mating in N. vitripennis occurs mostly between female and male offspring from the same maternal female directly after emergence from the host [97], or sometimes even within the host [94]. Contributions of natural and sexual selection to the evolution of premating reproductive isolation: a research agenda. However, above certain levels of parental dissimilarity, outbreeding depression can occur in hybrids, by genetic intrinsic incompatibility due to underdominance, chromosomal rearrangements or deleterious epistatic interactions, or extrinsic postzygotic barriers like maladaptation of hybrids to paternal habitats [120, 121]. As described above, wasp pupae were dissected from the host pupae and placed into Eppendorf tubes with perforated lids in groups of 10 female and four male pupae for eclosion and subsequent mating. J Stat Softw. Individual wasps were separated into Eppendorf tubes 15min prior to the experiments. A publication by Edward Mockford in 1997 recorded discovery of a new species of tiny wasp that is now known as the tiniest existing insect. In support of the identified population structure, Fst and Gst-values showed stronger differentiation between the microsatellite subpopulations but only low genetic differentiation between wasps from the two microhabitats. For experiments, single females were placed in Petri dishes ( 55mm) with five pupae of L. sericata each. 2014;9:e89214e89214. Quicke DLJ. in sympatry according to most definitions [5, 100]. Phylogeny and oscillating expression of period and cryptochrome in short and long photoperiods suggest a conserved function in Nasonia vitripennis. To further substantiate the hypothesis that this separation occurred in sympatry, the ecology and the population structure of N. vitripennis populations from Central Europe is required. 1995;10:5214. Thank you for reading. Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle. CR und RR performed host acceptance studies. Mol Ecol Notes. Ecol Evol. Annu Rev Ecol Evol Syst. 2019;19:216. Origin of wasps from bird nests or carrion is indicated above each graph. 1992;99:33557. Test statistics from olfactometer experiments with strains of Nasonia vitripennis from bird nests and carrion tested on the odour of bird nests or carrion. The females generally have an ovipositor which may be modified for sawing, piercing or stinging. 2017;35:31234. This website may contain names, images and voices of deceased Aboriginal and TorresStrait Islanderpeoples. These insects are used locally as a source of food by people in South America.

The biology of the parasitic wasp Mormoniella vitripennis [= Nasonia brevicornis](Walker). Takano S, Tuda M, Takasu K, Furuya N, Imamura Y, Kim S, et al. Trends Ecol Evol. Wallingford: CAB International; 1993. p. 2752. 2000;34:40137. Kopelman NM, Mayzel J, Jakobsson M, Rosenberg NA, Mayrose I. Clumpak: a program for identifying clustering modes and packaging population structure inferences across K. Mol Ecol Resour. The forewings and hindwings are held together by small hooks and the hindwings are smaller than the forewings and the wing venation (vein arrangement) is often much reduced. 2020;119:55966. The authors would like to thank Dick Askew and two unknown reviewers for very helpful comments and improving the English of the manuscript. Inbreeding effects in wild populations. Machado CA, Robbins N, Gilbert MTP, Herre EA. When winged, the wings form two membranous pairs that can be hooked together. Static 4-chamber olfactometer to test the reaction of female N. vitripennis to odours of bird nest and carrion. PubMed The odour field contained samples of bird nests or carrion. Box and whisker plots show minimum, maximum, 1st and 3rd quartile, and median. When these wasps failed to respond, all data from that day were discarded. Genetics. Considerations on speciation in Chalcidoidea (Hymenoptera). Prominent examples are ecologically divergent fish [10, 11] or phytophagous insects, which separated via host shift [12, 13]. However, because evidence so far indicates that this process is often the consequence of an interaction between sexual and natural selection [17,18,19,20,21] (but also see [22]) it was suggested to drop sexual selection as unique speciation mechanism [21]. Climate change affects the duration of the reproductive season in birds. 2012;28:25379. The primer mix contained two primer pairs with different fluorescent markers (HEX: Hexachloro-Fluorescein, 6FAM: 6-Carboxyfluoresceine, Applied Biosystems, Waltham, MA, US). Mol Ecol Resour. Google Scholar. Both flies and wasps are winged, they both tend to 'buzz' around in the garden, and sometimes in the house, and some species can cause irrit. You have reached the end of the main content. Otto SP, Whitton J. Polyploid incidence and evolution. Waser NM, Williams CF. 2001;16:38190. We used one individual wasp from each of the six laboratory strains N2, N3, N9, A1, A7, and A19 as well as 96 wasps (71 from bird nests and 26 from carrion) that had emerged from field-exposed host baits with alive fly pupae (see above) that were not used for further cultures. Psyche (Stuttg). Mol Ecol Resour. Annu Rev Entomol. Hedrick PW. Evolution. Genetics. Cryptic species are abundant [30] and new species are continuously being discovered (e.g. Grillenberger BK, Gadau J, Bijlsma R, Van De Zande L, Beukeboom LW. A F1-female offspring; B F1-male offspring; C F1-total offspring; D sex ratio. 2018;49:30330. The plots show minimum, maximum, 1st and 3rd quartile, median, outliers as circles and mean as asterisk. The plots show minimum, maximum, 1st and 3rd quartile, median, outliers as circles and mean as asterisk. 2018;8:906273. The number of strains from each of the habitats that significantly reacted to one of the two odours in the olfactometer experiments, and the distribution of individuals from the subpopulations between main and alternative microhabitat were analysed using Fisher Exact Test (Fig. Revision of the world species of the genus Chromoteleia Ashmead (Hymenoptera, Platygastridae, Scelioninae). J Anim Ecol. females mate only once [46, 47] and a second mating with non-related males after dispersal from the natal patch is rare. CAS Nosil P. Ecological speciation. Evans, H. E. & Ebarhard, J. W. 1970. Atalay D, Schausberger P. Balancing in-and out-breeding by the predatory mite Phytoseiulus persimilis. 1981;289:2733. Volumes 1-3. ZooKeys. Africanized Bees, or Killer Bees, are a major health threat to the population of the Southern United States, and are slowly expanding their geographic range. AC for the third barrier: AC3=RI3*[1(AC1+AC2)]. Sequential divergence and the multiplicative origin of community diversity. PM was financially supported by the Landesgraduiertenfrderung Baden-Wrttemberg. To establish inbreeding strains for laboratory cultures, wasps emerging from single host pupae from the bait bags were placed in Petri dishes with L. sericata pupae (approx. So far, there are no studies on reproductive barriers within one species of the Nasonia complex, e.g. S1, S2). Knig K, Krimmer E, Brose S, Gantert C, Buschlter I, Knig C, et al. Smithsonian Institution. 2014;40:5662. Prepared by the Department of Systematic Biology,Entomology Section, Biom J. To answer the question which reproductive barriers initiate speciation is difficult for two reasons. MH collected the field data. 2012;39:15880. 2005;59:31723. Fire Ants, of course, are a major concern in the Southern United States. J Insect Behav. Strains from different microsatellite clusters are also genetically distinct and separated by postmating isolation. Courtship behaviour by males was observed in all experimental couples, in which the male located the female (n=1059). Raw fragment length data were examined and the quality of individual signals/peaks checked with PeakScanner v1.0 (Applied Biosystems, Waltham, MA, US) according to [142]. In addition, subsequent Tukey-test showed that numbers of F1-female and F1-total offspring in couples from the same microsatellite cluster are significantly higher as compared to couples from different clusters, but that there is no difference in offspring numbers between couples from the same vs. couples from different microhabitats (Fig. 2014;105:78294. As described above for Chalcidoidea parasitoids in general, inbreeding is common in N. vitripennis. The Bee genera of North and Central America (Hymenoptera: Apoidea). Proc Natl Acad Sci. As numeric data from experiments onhost acceptance, i.e.

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